Human adrenomedullin (ADM) enzyme-linked kit price - Database & Sql Blog Articles

Human Adrenomedullin (ADM) Enzyme-Linked Immunoassay Kit Instructions for Use This kit is for research use only. Detection range: 2 ng/L -48 ng/L This kit is used to determine the content of adrenomedullin (ADM) in human serum, plasma and related fluid samples. Human Adrenomedullin (ADM) Enzyme Linked Kit Price Experiment Principle This kit uses the double antibody sandwich method to determine the level of human adrenomedullin (ADM) in the specimen. The microporous plate was coated with purified human adrenomedullin (ADM) antibody to prepare a solid phase antibody, and adrenomedullin (ADM) was sequentially added to the microcapsule of the coated monoclonal antibody, and then the HRP-labeled adrenal medulla was added. The ADM antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with adrenomedullin (ADM) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human adrenomedullin (ADM) in the sample was calculated from a standard curve. Kit composition 130 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 enzyme standard reagent 6ml × 1 bottle 8 standard product (96 ng / L) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes × 8 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 Bottle 12 sealed bag 1 person adrenomedullin (ADM) enzyme-linked kit price specimen requirements 1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase (HRP) activity. Procedure 1. Dilution of standard: This kit provides one original standard, which can be diluted in a small tube according to the following chart. 48 ng/L No. 5 Standard 150 μl of the original doubling standard Add 150 μl standard dilution 24 ng/L No. 4 standard 150 μl No. 5 standard Add 150 μl standard dilution 12 ng/L No. 3 standard 150 μl No. 4 Add 150 μl standard dilution 6 ng/L No. 2 standard 150 μl No. 3 standard Add 150 μl standard dilution 3 ng/L No. 1 standard 150 μl No. 2 standard Add 150 μl standard dilution 2. Add sample: Blank holes are respectively set (the blank control hole is not added with the sample and the enzyme standard reagent, and the other steps are the same), the standard hole, and the sample hole to be tested. Accurately load 50 μl of the standard on the enzyme-labeled plate, add 40 μl of the sample dilution to the well to be tested, and then add 10 μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, and shake gently to mix. 3. Incubation: The plate was sealed with a sealing film and incubated at 37 ° C for 30 minutes. 4. Dosing: Dilute 30 times concentrated washing solution with distilled water 30 times and reserve. 5. Wash: carefully remove the sealing film, discard the liquid, dry it, fill each well with washing liquid, let stand for 30 seconds and discard it. So repeat 5 times and shoot dry. 6. Add enzyme: 50 μl of enzyme labeling reagent was added to each well, except for blank wells. 7. Incubation: operation is the same as 3. 8. Washing: operation is the same as 5. 9. Color development: first add coloring agent A50μl to each well, then add coloring agent B50μl, gently shake and mix, avoid light and develop color at 37 °C 15 minutes. 10. Termination: 50 μl of stop solution was added to each well to terminate the reaction (when the blue color turned yellow). 11. Measurement: The absorbance (OD value) of each well was measured sequentially with a blank air conditioner of zero and a wavelength of 450 nm. The measurement should be carried out within 15 minutes after the addition of the stop solution. Summary of the price operation procedure of human adrenomedullin (ADM) enzyme-linked kit: Calculate the concentration of the standard as the abscissa and the OD as the ordinate. Draw the standard curve on the coordinate paper, and the standard curve according to the OD value of the sample. Find the corresponding concentration; multiply by the dilution factor; or calculate the linear regression equation of the standard curve using the concentration of the standard and the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply by the dilution factor, ie The actual concentration of the sample. Note 1. The kit should be taken out from the refrigerated environment and should be used at room temperature for 15-30 minutes before use. If the enzyme label is unsealed after opening, the slats should be stored in a sealed bag. 2. Concentrated washing liquid may crystallize out. When diluted, it can be heated and dissolved in a water bath. The washing will not affect the result. 3. The sampler should be used for each step, and the accuracy should be corrected frequently to avoid test errors. It is best to control the loading time within 5 minutes. If the number of specimens is large, it is recommended to use a gun. 4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the substance to be tested in the specimen is too high (the OD value of the sample is larger than the OD value of the first hole of the standard well), please first dilute the sample dilution with a certain multiple (n times) and then measure it. When calculating, multiply the total dilution by the total dilution. Multiple (×n×5). 5. The sealing film is intended for single use only to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the operation of the manual, the test results must be based on the reading of the microplate reader. All samples, washings and various wastes should be treated as infectious materials. 9. The different batch components of this reagent must not be mixed. 10. In the case of an English manual, the English manual shall prevail.